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The effects of three UV excimer laser radiations, with wavelengths of 193, 248 and 282 nm respectively, on the structure of chromatin (the complex of deoxyribonucleic acid with proteins that exists in eukaryotic cells nuclei) were investigated. The chromatin was extracted from livers of Winstar rats. The spectroscopic methods used are: fluorescence (Förster) resonance energy transfer (FRET), time...
The effect on fluorescence resonance energy transfer (FRET) of multiple labelling of DNA oligonucleotides with donor lanthanide chelate and acceptor CyDye™ fluors has been investigated. It is shown that using a multiple donor lanthanide chelate with a single acceptor Cy™ or Cy5 can increase sensitivity and fluorescence output. The enhanced FRET observed in the multiple donor label system has been...
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