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Halomonas pacifica CARE‐V15 was isolated from the southeastern coast of India to determine its genome sequence. Secondary metabolite gene clusters were identified using an anti‐SMASH server. The concentrated crude ethyl acetate extract was evaluated by GC–MS. The bioactive compound from the crude ethyl acetate extract was fractionated by gel column chromatography. HPLC was used to purify the 3,6‐diisobutyl‐2,5‐piperazinedione...