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A retroviral vector carrying herpes simplex virus thymidine kinase gene was constructed, and transfected into the ψ2 packaging cells. The replication-defective retrovirus produced by this cell line (ψ2tkn cells) was transduced into XC rat hepatoma cells, from which a cell line (XCtkn2) highly sensitive to ganciclovir was cloned. Ganciclovir suppressed the growth of XCtkn2 hepatoma and ψ2tkn cells...
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