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We probe endogenous NO production in WKY rats by trapping NO with iron–dithiocarbamate complexes. The aim was to detect non-stimulated NO production in small organs like kidneys of juvenile rats. The yields of mononitrosyl Fe–dithiocarbamate complexes are small and difficult to quantify in the presence of strong contaminating signals from Cu 2+ –DETC complexes. We evaluate four methods to...
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