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We have monitored real-time alterations in [Ca 2+ ] i in fluo-3-loaded cerebellar granule neurons exposed to domoate, and ascertained the influence of pharmacological blockers of various Ca 2+ entry pathways on intracellular Ca 2+ accumulation, excitatory amino acid (EAA) release and neuronal death. Domoate produced a rapid and concentration-dependent...
Considerable knowledge exists concerning the events mediating neuron death following a necrotic insult; prompted by this, there have now been successful attempts to use gene therapy approaches to protect neurons from such necrotic injury. In many such studies, however, it is not clear what sequence of cellular events connects the overexpression of the transgene with the enhanced survival. We do so,...
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