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In this study, we produce biodiesel from jatropha oil through enzymatic synthesis in a re circulated packed bed reactor (PBR). A solvent free system by three stepwise addition of one equivalent molar methanol in each step was chosen for this study. The enzyme was Lipozyme IM, a 1, 3 regiospecific immobilized lipase from Mucor miehei. Initially, we scrutinized the appropriate amount of lipase by varied...
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