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Using a double-labeling immunohistochemical and in situ hybridization technique for the simultaneous detection of tyrosine hydroxylase and preproenkephalin mRNA, we demonstrate that catecholaminergic and enkephalinergic neurons in the Kolliker-Fuse nucleus (K-F)/A7 region in the dorsolateral pons constitute separate populations. The enkephalinergic cell group is much larger than the catecholaminergic...
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