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Our studies show the influence of coumarins: xanthotoxin and o,o-dimethyl-fraxetin on apoptosis in human peripheral blood lymphocytes in vitro. Lymphocytes (derived by ethical protocol) were cultured for 72 h in vivo. The cell cultures were stimulated with xanthotoxin or o,o-dimethyl-fraxetin in various concentrations. Apoptotic cells (compaction and margination of nuclear chromatin, cytoplasmic condensation,...
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