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The feasibility of generating tomographic images of objects inside crude oil is investigated in this paper. Crude oil in the visible spectrum usually appears as black oil. Nevertheless, in the mid-infrared range, crude oil presents bands of high transmission. These windows of opportunities are explored to obtain data for generating cross-section images. In this paper, a first generation tomograph...
Objective-type total internal reflection fluorescence technique and Gaussian fit single particle tracking algorithm have been used to study the constrained Brownian motion characteristics of single fluorescent microspheres of 40 nm diameter in real-time. Three-dimensional tracking has been realized from the two-dimensional images by utilizing the exponential decay of the fluorescence intensity excited...
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