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To clone the members of ACC synthase gene family in sugarcane, two degenerate oligonucleotide primers were designed, coding for two conservative acid regons in ACC synthase protein family. PCR amplification was performed on sugarcane DNA template, which produced three fragments that were 1041 bp (Sc-ACS1), 1345bp (Sc-ACS2) and 1707bp (Sc-ACS3), respectively. Analyses by using the program of BLAST...
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