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Background Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address this...
Induction of an inter-connected microvessel network in a tissue-engineered construct prior to implantion may be an alternative to improve the success rate of cell/tissue survival and wound integration. Conditions of endothelial cell-seeding density and distribution were investigated in two 3-D angiogenesis culture systems. Endothelial cells were either seeded dispersed in a fibrin gel, or subconfluent...
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