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Interdigitated electrode based electrical biosensors are prominent in biosensor field. The large number of finger electrodes as comb structure gain high sensitivity through electrical measurements. Listeria monocytogenes is a serious food borne pathogen based bacterium that can cause dangerous disease to human, some infection may result in death. In here, biosensor was prepared from 1μm gap Aluminum...
This paper presents amino-terminated micro pattern transferring onto a mica substrate using polydimethylsiloxane (PDMS) soft stamp and proteoglycan (PG) molecular immobilization. PDMS bump-arrays with ultra-smooth surface were fabricated utilizing anisotropic etched silicon substrate as a mold. Coating and removal of releasing-agents were investigated to improve detachability of PDMS stamp from silicon...
Immobilization is an important process of biosensor application. The simplest functionalize diamond surface was done by attached biomolecule on diamond surface with chemical linker compound modification. Carboxyl treatment is one choice for diamond functionalization. Methane gas was diluted with hydrogen gas for CH3 termination as an initial functionalization of diamond to transform to carboxyl termination...
This paper presents an overview of the quartz crystal microbalance sensor (QCM) for bacteria application. The QCM sensor can be considered as a representative mechanical transducer for a biosensor device that converts the mass of target analytes into an electrical signal. Microorganisms' detection using QCM sensor can be applied in applications in the field of environment, biomedical area, and food...
This paper presents a novel Ni-Co alloy coated QCM biosensor fabricated by electroplating nickel and cobalt on the surface of 10-MHz AT-cut QCM. The biotin was immobilized on the coated surface by the affinity mechanism of Ni-Co alloy. Then, the immunoreactions can be performed on the QCM biosensors. The results show that the Ni-Co alloy coated QCM biosensor can provide specific binding with His-tagged...
Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266 nm light yields the pi-pi* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with...
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