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Organotypic slice cultures were established as a model that own properties of both cell culture and animal model. The most often used slice culture is derived from hippocampus but depending on the part of brain affected with pathology, researchers established cultures from cerebellum, midbrain or striatum. Above mentioned models allowed the investigation of disorders resulting from e.g. ischemia,...
The study investigated the anatomy of the arteries of the brain in seven donkeys following intravascular injection of colored latex via the a. vertebralis and a. carotis communis. Arterial blood washed the brain via the bilateral a. carotis interna and a. vertebralis and via the unpaired a. basilaris. A. carotis interna entered the cavum cranii at the level of sulcus pontocruralis, and ramified at...
Poly(ADP-ribose) polymerase (PARP) is a conserved enzyme involved in the regulation of DNA repair and genome stability. The role of PARP during aging is not well known. In this study PARP activity was investigated in nuclear fractions from hippocampus, cerebellum, and cerebral cortex of adult (4 months), old adult (14 months) and aged (24-27 months) rats. Concomitantly, the free radical evoked lipid...
Ca2+-ATPase, the enzyme responsible for maintenance of low resting Ca2+ level in the nerve cell, has been purified from rat cortical, cerebellar and hippocampal synaptosomal membranes by affinity chromatography on Calmodulin-Agarose which followed Reactive Red 120-Agarose column. The enzymes from these three regions ran as broad, monomeric bands on SDS-PAGE with a molecular weight at range 130-138...
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