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Abstract. Because changes in cell volume might disturb the normal function of animal cells, most cells are endowed with volume-regulating mechanisms. Experimentally induced changes in cell volume are often paralleled by changes in the membrane potential, which might affect a variety of transport processes across the cell membrane and, in turn, volume regulation[1, 2]. We have shown previously that...
Changes in cell volume can be recorded by a potentiometric method based on ion-selective microelectrodes filled with the classical K + exchanger Corning 477317, which is very sensitive to large quaternary ammonium ions (PNAS 82 (1985) 6014; Electrochim. Acta 47 (2001) 309). We investigated the effect of hyperosmotic bathing solutions on cell volume, the intracellular concentrations of Na ...
A potentiometric method to monitor cell volume changes in intact biological tissues is based on ion-selective microelectrodes filled with the classical K + exchanger Corning 477317, which is very sensitive to large quaternary ammonium ions. After introduction into a single cell, these cations can be used as volume marker by relating their changes in concentration to changes in cell volume,...
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