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Bio-indigo was produced in 3000-L batch and 5-L continuous fermentation of a recombinant Escherichia coli DH5α harboring a novel gene encoding flavin-containing monooxygenase (FMO). Batch fermentation in a 3000-L fermenter produced 911±22mgL −1 of indigo from 2gL −1 tryptophan as a substrate (yield 46.9%) under the following culture conditions as follows: culture temperature 30°C,...
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