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Fast and accurate profiling of exogenous gene expression in host cells is crucial for studying gene function in cellular and molecular biology, but still faces the challenge of incomplete co‐expression of reporter genes and target genes. Here, a single‐cell transfection analysis chip (scTAC) is presented, which is based on the in situ microchip immunoblotting method, for rapid and accurate analysis...
In article number 2101108, Haiyang Xie, Xianting Ding, and co‐workers report a single‐cell‐transfection analysis chip (scTAC) based on highly sensitive light‐fixed hydrogels. The scTAC is used for rapid, high‐throughput single‐cell screening of transfected cells by in situ immunoblotting methods to assess plasmid constructs and exogenous gene expression in host cells. The developed system is able...
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