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This work presents a microfluidic chip with a 96-cell-trap array (16-row × 6-column) for immobilization and comparative culturing of single yeast cells. In this microfluidic system, budding yeast (S. cerevisiae) cells can be delivered to each column of the cell-trap array by means of a laminar focused flow and be reliably captured at the traps in each column through hydrodynamic forces. Immobilized...
A novel microfluidic chip with simple design, easy fabrication and low cost, coupled with high-sensitive laser induced fluorescence detection, was developed to provide continuous single-cell analysis based on dynamic cell manipulation in flowing streams. Making use of laminar flows, which formed in microchannels, single cells were aligned and continuously introduced into the sample channel and then...
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