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We characterized membrane ionic currents underlying the action potential in single myocytes freshly isolated from the stroma of the guinea pig prostate. Whole cell and single channel currents were recorded in single stromal smooth muscle cells using standard patch clamp techniques. A rapidly activating, nifedipine (1 μM) sensitive Ca 2+ current was recorded in CsCl (130 mM) filled myocytes...
Stretch activated nonselective cationic channels (SACs) are present in urinary bladder myocytes and thought to be activated during bladder filling. We investigated the relationship of stretch induced calcium signaling inhibition in bladder myocytes and bladder compliance modulation in an in vitro whole bladder model. Grammostola spatulata venom (SpiderPharm, Yarnell, Arizona) was purified by preparative...
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