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Due to their oftentimes ambiguous nature, phosphopeptide positional isomers can present challenges in bottom‐up mass spectrometry‐based workflows as search engine scores alone are often not enough to confidently distinguish them. Additional scoring algorithms can remedy this by providing confidence metrics in addition to these search results, reducing ambiguity. Here we describe challenges to interpreting...
The phosphorylation of photosystem II (PSII) and its antenna (LHCII) proteins has been studied, and its involvement in state transitions and PSII repair is known. Yet, little is known about the phosphorylation of photosystem I (PSI) and its antenna (LHCI) proteins.
Here, we applied proteomics analysis to generate a map of the phosphorylation sites of the PSI–LHCI proteins in Chlorella ohadii cells...