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The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities...
The aim of these investigations was to prepare the antigen and control sera for the ELISA. Positive control sera were obtained from 163 cows showing clinical symptoms of H. bovis infection and negative control sera came from 60 cows free from the infection. The antigen for the ELISA was prepared from H. bovis L1 larvae. Three basic fractions were isolated from whole excretory/secretory antigen by...
The prevalence of bovine viral diarrhea (BVD) virus infection on the basis of serological testing of cattle has been evaluated. Blood samples were analyzed in micro-seroneutralization test for the presence of BVD virus neutralizing antibodies against 1000 TCID₅₀ of NADL reference strain. Neutralizing antibodies were found in 83 per cent of animals tested. The percentage of seropositive animals was...
A study on the seroprevalence of FMD antibodies in cattle in the „ring” vaccination zone was performed. Out of 550 samples of sera examined by LPB ELISA, 240 samples (44%) have been found positive to FMDV type A,O and/or C (ELISA titres > log 1.6). The highest Ievel of antibodies was found for FMDV type O₁BFS; about 10% of tested sera had titres > log 3.01. A half-life of maternal antibodies...
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