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This paper describes an original method to detect XFP-tagged proteins in time-lapse microscopy. Non-local measurements able to capture spatial intensity variations are incorporated within a Conditional Random Field (CRF) framework to localize the objects of interest. The minimization of the related energy is performed by a min-cut/max-flow algorithm. Furthermore, we estimate the slowly varying background...
Membrane-bound macromolecules are responsible for structural support and mediation of cell-cell adhesion in tissues. Quantitative analysis of these macromolecules provides morphological indices for damage or loss of tissue, for example as a result of exogenous stimuli. From an optical point of view, a membrane signal may have nonuniform intensity around the cell boundary, be punctate or diffused,...
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