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Apoptotic programmed cell death (PCD) is a common and fundamental aspect of developmental maturation. Image processing techniques have been developed to detect apoptosis at the single-cell level in a single still image, while an efficient algorithm to automatically analyze the temporal progression of apoptosis in a large population of cells is unavailable. In this work, we have developed an ImageJ-based...
We have proposed a novel handheld healthcare platform, combining a pocket-sized colorimetric reader (13.5 × 6.5 × 2.5 cm3) and commercially available 10-parameter urinalysis paper strips (glucose, protein, glucose, bilirubin, urobilinogen, ketones, nitrite, pH, specific gravity, erythrocytes, and leukocytes), capable of sending data with a smart phone (Samsung electronics co., Omnia 2) using a window...
Microscopy-based diagnosis of certain diseases or infections, e.g. with human papilloma viruses (HPV) for the identification of high risk patients for cervical cancer, relies more and more often on immunocytochemical marker stains. These markers stain cells that exhibit a particular protein. In addition to one or more marker stains, pathologists need to simultaneously assess the morphology of the...
Detection of change in microenvironmental pH may be essential for analyzing biological processes, if transitory biodegradable scaffold materials are used for tissue engineering in bone regeneration. In this study, we created a pH indicator immobilized-gel-sheet for pH measurement. By using gel-sheet, we can investigate the local microenvironment of biodegradable materials, such as octacalcium phosphate...
The ovary is a female organthat houses a fixed supply of germ cells (oocytes). The absolute number of oocytes at any given stage can be a useful indicator of fertility. Obtaining accurate assessments of the oocyte reserve in humans and experimental models can be time consuming and error prone. In this paper a new approach to facilitate oocyte counting in microscope images of mouse ovariesis presented...
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