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A detailed understanding of subcellular structure motility is critical to understanding how cells regulate the delivery of specific proteins from the site of synthesis to the site of action. Diverse modalities have been observed for subcellular dynamics, such as directional movement, random movement, tethered movement, object appearing, and disappearing. Motion modality detection is important for...
The Human Protein Atlas is a rich source of location proteomics data. In this work, we present an automated approach for processing and classifying major subcellular patterns in the Atlas images. We demonstrate that two different classification frameworks (support vector machine and random forest) are effective at determining subcellular locations; we can analyze over 3500 Atlas images with a high...
Elastin-Like Polypeptides are an interesting class of recombinant proteins that mimic elastin. Here we present some data about phase transition properties and particle formation features of the recombinantly expressed Human Elastin-Like polypeptide (HELP). This macromolecule shows a promising potential as vehicle for delivery and controlled release of active compounds.
Epidermal growth factor receptor (EGFR) is an important cell surface protein that's over-expressed in many human cancer cells. We designed a novel peptide ligand (named D4) based on the crystal structure of EGFR that can bind to a surface pocket on EGFR. D4-PEG-lipid conjugates were made and used to modify liposome surface. Confocal fluorescence microscopy data showed that D4-liposome (D4-RL) bound...
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