The asidine darkling beetles (Coleoptera: Tenebrionidae: Asidini) are a morphologically diverse tribe of flightless tenebrionids found in many arid and sub‐arid habitats around the world. The 260 currently described North American species are contained in 27 genera, all of which are restricted to the western half of the continent. Evolutionary relationships within and between the North American Asidini (Coleoptera: Tenebrionidae) genera were reconstructed using partial sequences of mitochondrial cytochrome c oxidase I (COI) (660 bp) and nuclear ribosomal 28S (D2 region) (492 bp), and 100 phenotypic characters for 50 North American asidine species, representing 20 of the 27 currently described genera and 1 new genus. Species from two additional tenebrionid tribes (Branchini and Coniontini) and the South American asidine genus Cardigenius were chosen as outgroups. Analyses were performed using maximum parsimony and Bayesian inference methods. Clade support was inferred based on the posterior probability distribution of tree topologies, nonparametric bootstrap analysis, and partitioned Bremer support indices. The generic classification of the North American Asidini is revised based on the results, with ten genera recognized. Seven current genera: CraniotusLeConte, HeterasidaCasey, LitasidaCasey, MicroschatiaSolier, PelecyphorusSolier, PhilolithusLacordaire, StenomorphaSolier, are redescribed, and ArdamimicusSmith gen.n., FerveoventerSmith gen.n. and MicrasidaSmith gen.n. are described, including Ardamimicus cognatoiSmith sp.n., Ferveoventer browniSmith sp.n. and Micrasida obrienorumSmith sp.n. Twenty current genera are treated as subgenera pending further analyses: PhilolithusLacordaire with subgenera GlyptasidaCasey, GonasidaCasey, HerthasidaWilke and TisamenesChampion; PelecyphorusSolier, with subgenera: AstrotusLeConte, ParasidaCasey (= Plesiasidanom.n), PoliorcetesChampion, SicharbasChampion, StenosidesSolier, UcalegonChampion and ZaleucusChampion, and StenomorphaSolier with subgenera AsidinaCasey, AsidopsisCasey, BothrasidaCasey, MegasidaCasey, NotiasidaCasey, PlatasidaCasey, PycnomorphaMotschulsky, StethasidaCasey and TrichiasidaCasey; all stat.rev.