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Aerolysin‐based direct analysis of oligonucleotides with secondary structure (e.g., G‐quadruplex) remains a challenge. In article number 1704520, Yi‐Lun Ying, Yi‐Tao Long, and co‐workers present a strategy employing an aerolysin nanopore to analyze structured oligonucleotides that are extended to 30 bases in length by a cation‐regulation mechanism. This method can in principle be applied to genetic...
Current‐induced concentration polarization of nanoporous media is explored theoretically by using approach of local thermodynamic equilibrium within nanopore cross‐sections. The problem is solved in quadratures in terms of irreversible thermodynamics. The phenomenological coefficients are further specified by using capillary space‐charge model for straight slit‐like and cylindrical capillaries. This...
An aerolysin nanopore is employed as a sensitive tool for single‐molecule analysis of short oligonucleotides (≤10 nucleotides), poly(ethylene glycol) (PEGs), peptides, and proteins. However, the direct analysis of long oligonucleotides with the secondary structure (e.g., G‐quadruplex topology) remains a challenge, which impedes the further practical applications of the aerolysin nanopore. Here, a...
The controlled functionalization of surfaces with proteins is crucial for many analytical methods in life science research and biomedical applications. Here, a coating for silica‐based surfaces is established which enables stable and selective immobilization of proteins with controlled orientation and tunable surface density. The coating is reusable, retains functionality upon long‐term storage in...
DNA folding is not desirable for solid‐state nanopore techniques when analyzing the interaction of a biomolecule with its specific binding sites on DNA since the signal derived from the binding site could be buried by a large signal from the folding of DNA nearby. To resolve the problems associated with DNA folding, ionic liquids (ILs), which are known to interact with DNA through charge–charge and...
Controlling the molecular interactions through protein nanopores is crucial for effectively detecting single molecules. Here, the development of a hetero‐oligomeric nanopore derived from Nocardia farcinica porin AB (NfpAB) is discussed for single‐molecule sensing of biopolymers. Using single‐channel recording, the interaction of cyclic oligosaccharides such as cationic cyclodextrins (CDs) of different...
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