This study aimed to investigate the effects of 24 h total sleep deprivation on serum rennin, angiotensin II, cortisol, dopamine, epinephrine and norepinephrine in servicemen. A total of 223 servicemen had blood drawn before and after deprivation for serum rennin, angiotensin II and cortisol measurements. Altogether 60 participants were randomly selected from all the participants as a subsample for serum dopamine, epinephrine and norepinephrine measurements. The results revealed that the levels of all six hormones after sleep deprivation were significantly higher than those before. We conclude that sleep deprivation probably serves as an endogenous stressor via complex pathways.