Rationale
The key to successful experiments in matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) is to apply the matrix uniformly to the sample. With the development of automated equipment, uniform matrix application has made great progress while the sample preparation required to acquire a better image becomes complicated.
Methods
The approach is to apply the matrix uniformly to tape and adhere it to the tissue section. We call this the sheet‐enhanced technique (Set) method.
Results
The Set method promotes ionization of biomolecules as well as the spray method. This procedure does not require the preparation and application of a matrix solution for each experiment, dramatically reducing the time and effort of matrix deposition.
Conclusions
In the present study, we have developed the Set method as a new matrix application method. The method promotes ionization of biomolecules as well as the spray method for MALDI‐IMS.