Social‐evaluative threat evokes increases in salivary cortisol and heightened cardiovascular reactivity. However, the types or content of social threats underlying these physiological responses are not clearly delineated in direct comparisons. Based in interpersonal theory, the present study manipulated high and low levels of acceptance threat (i.e., evaluation of likability, potential for inclusion) and status threat (i.e., evaluation of competence, leadership potential) during a modified Trier Social Stress Test, using a sample of 137 undergraduates (73 women). Both acceptance threat and status threat heightened salivary cortisol, heart rate, and blood pressure responses to the task. Hence, concerns about social inclusion or connection with others and concerns about social standing or status can contribute independently to physiological stress responses.