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BACKGROUNDNext‐generation sequencing (NGS) technologies offer tremendous possibilities for accurate detection of mutations endowing pesticide resistance, yet their use for this purpose has not emerged in crop protection. This study aims at promoting NGS use for pesticide resistance diagnosis. It describes a simple procedure accessible to virtually any scientist and implementing freely accessible programs...
BACKGROUND
Next Generation Sequencing (NGS) technologies offer tremendous possibilities for high‐throughput pesticide resistance diagnosis via massive genotyping‐by‐sequencing. Herein, we used Illumina sequencing combined with a simple, non‐commercial bioinformatics pipe‐line to seek mutations involved in herbicide resistance in two weeds.
RESULTS
DNA was extracted from 96 pools of 50 plants for...
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