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BACKGROUND
RNA interference combined with digital gene expression (DGE) analysis can be used to study gene function. Trehalose‐6‐phosphate synthase (TPS) plays a key role in the synthesis of trehalose and insect development.
RESULTS
DGE analysis revealed that the expression of nine or four chitinase genes was reduced significantly 48 h after NlTPS1 and NlTPS2 knockdown by RNAi, respectively. Additionally,...
BACKGROUND
Two RNA silencing pathways in insects are known to exist that are mediated by short interfering RNAs (siRNAs) and microRNAs (miRNAs), which have been hypothesised to be promising methods for insect pest control. However, a comparison between miRNA and siRNA in pest control is still unavailable, particularly in targeting chitin synthase gene A (CHSA).
RESULTS
The dsRNA for Nilaparvata lugens CHSA...
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