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N‐acetylated sugars are often found, for example, on the lipopolysaccharides of Gram‐negative bacteria, on the S‐layers of Gram‐positive bacteria, and on the capsular polysaccharides. Key enzymes involved in their biosynthesis are the sugar N‐acetyltransferases. Here, we describe a structural and functional analysis of one such enzyme from Helicobacter pullorum, an emerging pathogen that may be associated...