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Coelenterazine‐v (CTZ‐v), a synthetic derivative with an additional benzyl ring, yields a bright bioluminescence of Renilla luciferase and its “yellow” mutant with a significant shift in the emission spectrum toward longer wavelengths, which makes it the substrate of choice for deep tissue imaging. Although Ca2+‐regulated photoproteins activated with CTZ‐v also display red‐shifted light emission,...