The transmission of airborne diseases in animals poses great risks to animal safety with potential significant economic losses. In this study, we report on the use of a dielectric barrier discharge (DBD) for in‐flight inactivation of an airborne aerosolized porcine reproductive and respiratory syndrome virus. The infectivity of the sampled virus downstream compared to upstream of the DBD reactor as determined by the TCID50 method showed a ∼3.5 log10 reduction in the virus titer. Independent testing of the viral genome by the reverse‐transcription quantitative real‐time polymerase chain reaction method confirmed the inactivation with minimal filtering effects. Both short‐lived species such as and and peroxynitrous acid (ONOOH) chemistry at low pH in the virus‐laden droplets are suggested to be responsible for the observed inactivation.