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Stable isotope labeling techniques hold great potential for accurate quantitative proteomics comparisons by MS. To investigate the effect of stable isotopes in vivo, we metabolically labeled high anxiety‐related behavior (HAB) mice with the heavy nitrogen isotope 15N. 15N‐labeled HAB mice exhibited behavioral alterations compared to unlabeled (14N) HAB mice in their depression‐like phenotype. To correlate...
Several techniques based on stable isotope labeling are used for quantitative MS. These include stable isotope metabolic labeling methods for cells in culture as well as live organisms with the assumption that the stable isotope has no effect on the proteome. Here, we investigate the 15N isotope effect on Escherichia coli cultures that were grown in either unlabeled (14N) or 15N‐labeled media by LC‐ESI‐MS/MS‐based...
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