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In Arabidopsis, the C‐repeat binding factors (CBFs) have been extensively studied as key transcription factors in the cold stress response. However, their exact functions in the cold response remains unclear due to the lack of a null cbf triple mutant.
In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T‐DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3...
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