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The hyperpolarization of metabolic substrates at low temperature using dynamic nuclear polarization (DNP), followed by rapid dissolution and injection into an MRSI or NMR system, allows in vitro or in vivo observation and tracking of biochemical reactions and metabolites in real time. This article describes an elegant approach to sample preparation which is broadly applicable for the rapid polarization...
In this study, a mixture of pyruvic acid and the perfusion agent HP001 was co‐polarized for simultaneous assessment of perfusion and metabolism in vivo. The pre‐polarized mixture was administered to rats with subcutaneous MDA‐MB‐231 breast cancer xenografts and imaged using an interleaved sequence with designed spectral–spatial pulses and flyback echo‐planar readouts. Voxel‐by‐voxel signal correlations...
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