Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells, with applications in neuroscience and clinical use for treatment of neurodegenerative disease and neurological disorders. NSC are obtained by isolation from tissue, or differentiated from pluripotent cells. In this chapter, we have described several ways to culture human neural stem cells. The culture context can be varied and selected based on the need for study. Human neural stem cells can be proliferated either as adherent monolayer culture or a suspension culture of neurospheres. For adherent culture, the adherent factor of Geltrex, mouse Laminin or xeno free material of CellStart, Vitronectin or human laminin can be selected. In terms of medium context, supplement and growth factors can be selected based on the research purpose. We describe the steps in detail to prepare matrix, culture medium, and procedures to support neural stem cells.