Scope
Myricetin is a dietary flavonol and widely distributed in many edible plants. It has been reported to have many bioactivities and considered as a promising chemopreventive compound. The present study aimed to investigate the influences of myricetin on gene expressions in genome‐wide and underlying mechanisms.
Methods and results
Among total 44K gene probes, myricetin treatment upregulated the signals of 143 gene probes (0.33% of total probes) and downregulated signals of 476 gene probes (1.08% of total probes) by greater than or equal to twofold in HepG2 cells. The network pathway analysis revealed that nuclear factor (erythroid‐derived 2)‐like 2 (Nrf2)‐mediated antioxidant response element (ARE) activation is involved in myricetin‐induced genes expressions. Molecular data revealed that myricetin activated Nrf2‐ARE pathway by inhibiting Nrf2 ubiquitination and protein turnover, stimulating Nrf2 expression and kelch‐like erythroid cell‐derived protein with CNC homology (ECH)‐associated protein 1 modification. All of these events finally increased nuclear Nrf2 accumulation and ARE‐binding activity to enhance ARE‐mediated genes expressions. Additionally, treatment with Nrf2 small interfering RNA attenuated the myricetin‐induced ARE activity and gene expression.
Conclusion
An Nrf2‐mediated ARE activation is involved in myricetin‐induced expression profiling in hepatic cells.