Novel polyureas are synthesized from lysinyl residues. The polyurea thus prepared yields a durable self‐standing membrane that can be converted into a molecular recognition material by using Z‐D‐Glu or Z‐L‐Glu as a print molecule. The Z‐D‐Glu molecularly imprinted membrane adsorbes the D‐isomer of Glu in preference to the corresponding L‐Glu and vice versa. Even though the polyurea consists of L‐lysinyl residues, both Z‐D‐Glu and Z‐L‐Glu work as print molecules to construct molecular (chiral) recognition sites in the membrane. Those molecularly imprinted membranes show chiral separation abilities when a concentration gradient or an applied potential difference is applied as a driving force for membrane transport.