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Cellular activities in 3D are differentially affected by several matrix‐intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD‐alginate hydrogels showed steady‐state levels of metabolic activity and were in a nearly non‐proliferative...
We report the encapsulation of three different proteases in bioinspired silica. Silica particles were formed under mild reaction conditions using cationic amine‐rich ethyleneamines as initiators, which resulted in aggregations of nanoscale spheres. Following encapsulation, the proteases were characterized for their hydrolytic and aminolytic activities. The encapsulation resulted in an increase in...
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