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Recycling counter‐current chromatography (CCC) together with step‐gradient CCC and medium‐pressure liquid chromatography (MPLC) was employed to separate nine anthraquinone compounds from Cassia obtusifolia L. in this study. The results showed that recycling CCC is a powerful tool for compounds that are difficult to separate with common elution mode. CCC was the better option for crude material while...
A sensitive and reliable ultra‐high performance liquid chromatography–electrospray ionization–tandem mass spectrometry has been developed and partially validated to evaluate the quality of Semen Cassiae (Cassia obtusifoliaL.) through simultaneous determination of 11 anthraquinones and two naphtha‐γ‐pyrone compounds. The analysis was achieved on a Poroshell 120 EC‐C18 column (100 mm × 2.1 mm, 2.7 μm;...
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