Saffold virus (SAFV) is a newly discovered human virus which is classified into the genus Cardiovirus of the family Picornaviridae. A total of 608 fecal specimens collected during January 2012 to December 2013 from children with diarrhea in Chiang Mai, Thailand were investigated for SAFV by RT‐nested PCR and sequence analysis. Of these, nine out of 608 (1.5%) were positive for SAFVs and four genotypes were identified, SAFV1, SAFV2, SAFV3, and SAFV4. SAFV mono‐infection was found in five cases (CMH‐S038–12, CMH‐S071–12, CMH‐S102–12, CMH‐N029–12, and CMH‐S048–13), while co‐infection with other viruses causing diarrhea was observed in four cases (CMH‐S021–12, CMH‐S115–12, CMH‐N048–13 and CMH‐N103–13). This study provides more information about the genetic background of SAFV circulating in pediatric patients with diarrhea in Thailand. J. Med. Virol. 87:702–707, 2015. © 2015 Wiley Periodicals, Inc.