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We demonstrate the development of a mass spectrometry‐based epitope‐mapping procedure in combination with Western blot analysis that works also with antigens that are insoluble in nondenaturing buffers consuming minute amounts of antigen (approximately 200 pmol) and antibody (approximately 15 pmol), respectively. A polyclonal anti‐TRIM21 rabbit antibody serum is applied as a model serum for future...
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