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Aims: To assess methods for both measurement of and comparison between fibril spacing arrangement in transmission electron micrograph (TEM) images of collagen fibrils. Methods: Rabbit corneas were glutaraldhyde fixed, treated with osmium tetroxide and then thin sections stained with aqueous phosphotungstic acid. TEM images, at 28,000 magnification, of collagen fibrils in cross section were projected...
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