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This study aimed to investigate the cell cycle arrest and autophagy induced by iron overload in MC3T3‐E1 cells. MC3T3‐E1 cells were cultured in different concentrations of ferric ammonium citrate (FAC), and Perls’ Prussian blue reaction was used to detect the iron levels of the cells. CCK‐8 assays were used to detect the growth of MC3T3‐E1. The level of reactive oxygen species (ROS) within cells...
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