Background
Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is the causative agent of coronavirus disease 2019 (COVID‐19) and is capable of human‐to‐human transmission and rapid global spread. Thus, the establishment of high‐quality viral detection and quantification methods, and the development of anti‐SARS‐CoV‐2 agents are critical.
Methods
Here, we present the rapid detection of infectious SARS‐CoV‐2 particles using a plaque assay with 0.5% agarose‐ME (Medium Electroosmosis) as an overlay medium.
Results
The plaques were capable of detecting the virus within 36–40 h post‐infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS‐CoV‐2 in a time‐ and concentration‐dependent manner.
Conclusions
These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS‐CoV‐2.