Background
We propose a simple, sensitive, and fast high‐performance liquid chromatography ultraviolet detection (HPLC‐UV) method for the quantitative determination of bosutinib in human plasma.
Methods
Plasma samples were processed using an Oasis hydrophilic‐lipophilic balance extraction cartridge (1 mL, 30 mg). Bosutinib and the internal standard imatinib were separated using a mobile phase of 0.5% Na2PO4H2O (pH 3.5)‐acetonitrile‐methanol (55:25:20, v/v/v) on a CAPCELL PAK C18 MG II reversed‐phase column 250 nm×4.6 nm i.d., at a flow rate of 1.0 mL/min, with ultraviolet detection at 250 nm.
Results
The calibration curve exhibited linearity over the bosutinib concentration range of 25‐1500 ng/mL at 250 nm, with coefficient of variation for intraday precision of 2.42%, 6.04%, and 1.11% for 100, 250, and 1500 ng/mL, respectively, of bosutinib. The lower limit of detection was 20 ng/mL. The extraction recovery rates for bosutinib ranged from 84.36% to 85.82%. The intra‐ and interday precision was below 8.7%, and the accuracy ranged from −5.95% to 5.85% over the linear range. No notable matrix effects or astaticism were observed.
Conclusion
The proposed HPLC‐UV method was successfully applied as an assay to detect bosutinib in human plasma.