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Using short hairpin RNA against p53, transient ectopic expression of wild‐type p53 or mutant p53 (R248W or R175H), and a p53‐ and p21‐dependent luciferase reporter assay, we demonstrated that growth arrest and apoptosis of FaDu (human pharyngeal squamous cell carcinoma), Hep3B (hepatoma), and MG‐63 (osteosarcoma) cells induced by aloe‐emodin (AE) are p53‐independent. Co‐immunoprecipitation and small...
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