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Nanos2 is an evolutionarily conserved RNA‐binding protein containing 2 CCHC‐type zinc finger motives. Here, we report that Nanos2 is strongly expressed in the testis compared to other tissues in chicken (Gallus gallus). Overexpression and knockout plasmid vectors were constructed, and in‐vitro Cas9/gRNA digestion and T7 endonuclease I (T7E1) assay indicated that Nanos2‐g1 possessed the highest knockout...
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