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Understanding the mechanisms of structural organization of corneal stroma is scientifically interesting and may lead to advances in corneal tissue engineering. In the past, we used the combination of second harmonic generation and fast Fourier transform to study a number of topics including the structural alteration of human keratoconus. Recently, we applied this approach to studying changes of chick...
Collagen fibers play an important role in both the structure and function of various tissues in the human body. Visualization and quantitative measurements of collagen fibers are possible through imaging modalities such as second harmonic generation (SHG), but accurate segmentation of collagen fibers is difficult for datasets involving variable imaging depths due to the effects of scattering and absorption...
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